Overcoming Chiral Method Development Challenges: UPLC-MS/MS Method Development for Determination of Dextroamphetamine and Levoamphetamine in Human Plasma after Chiral Derivatization - April, 2014

Enantiomeric determination of dextro-/levo- (d-/l-) amphetamines is of great interest to both forensics (amphetamine and its analogues are the most abused drugs) and amphetamine containing new drug development because the two enantiomers may behave very differently in pharmacokinetic and pharmacologic activities. Existing methods use GS/MS after derivatization to form diastereomers, or HPLC/MS using chiral stationary phase without derivatization or nonchiral stationary phase after chiral derivatization. These methods either require relatively long analysis time (> 10 mins) or can achieve LLOQ of only 1 ng/mL. The following poster abstract outlines the method development challenges and solutions applied for our UPLC-MS/MS method to reliably measure both d- and l-amphetamines of 0.1 to 100 ng/mL in human plasma within run time of ~4.5 minutes: 

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