DMPK

Ultracentrifugation for Protein Binding Determination - November, 2013

Equilibrium dialysis has been the gold standard for protein binding determinations. However, this method has its limitations particularly for compounds with very high non-specific binding, plasma instability, or inability to diffuse through the dialysis membrane. Ultracentrifugation may be an alternative method for these types of compounds.

A recent poster communication by QPS at ISSX demonstrated the potential advantage of ultracentrifugation. Ultracentrifugation can be useful for assessing plasma protein binding of compounds with high molecular weight or high non-specific binding.

To view the full poster communication, please navigate to: 
http://www.qps.com/posters/

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