DMPK

Metabolite Profiling Using Human Hepatocyte Co-cultures and UHPLC-Q-TOF-MS with Data Independent MS/MS - April, 2014

The identification of human derived metabolites is an imperative step in drug discovery and development process. Typical studies are conducted in human in vitro systems and metabolites are identified utilizing HPLC-MS/MS. Human hepatocyte co-culture is a new in vitro system, which is comprised of a mixture of hepatocytes and fibroblast cells, capable of remaining viable and highly functional for up to 4 weeks. In this poster abstract, we present the application of high resolution mass spectrometry (HRMS), ultrahigh-pressure liquid chromatography (UHPLC), full scan acquisition, and Sequential Window acquisition of All Theoretical fragment ion spectra (SWATH™) which were used to profile and characterize metabolites of diclofenac, linezolid, and ziprasidone following incubations in human hepatocyte co-cultures.

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