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Biotransformation

Home / Biotransformation
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Biotransformation

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All Biotransformation studies are done in the QPS DMPK labs in Newark, Delaware, USA

Preclinical biotransformation studies play a crucial role in drug development. Biotransformation refers to the chemical alteration of compounds within an organism, often through processes like metabolism. In the context of drug development, preclinical biotransformation studies involve investigating how a drug candidate is metabolized in laboratory animals before advancing to clinical trials in humans.

Key aspects of preclinical biotransformation studies include:

1. Metabolism Assessment: Researchers analyze how the drug candidate is metabolized within the animal model. This helps predict how the drug may be metabolized in humans and identifies potential metabolites.

a. Objective: Understand how the drug candidate is metabolized in laboratory animals.

b. Methods: Analyze biological samples (blood, urine, tissues) to identify metabolites and quantify parent drug levels.

c. Significance: Predict how the drug may be metabolized in humans, providing insights into potential pathways and facilitating the design of subsequent studies.

2. Identification of Metabolites: Identification of metabolites is essential for understanding the potential impact on the efficacy and safety of the drug. Some metabolites may be pharmacologically active, while others may contribute to toxicity.

a. Objective: Identify and characterize metabolites generated during drug metabolism.

b. Methods: Use analytical techniques such as mass spectrometry and chromatography to separate, identify, and quantify metabolites.

c. Significance: Determine the nature of metabolites (active, inactive, toxic) to assess their impact on drug efficacy and safety.

3. Species Comparison: Studying biotransformation across different species is important to assess the relevance of animal models to human metabolism. Humans and animals may metabolize drugs differently, and understanding these differences is crucial for predicting human outcomes.

a. Objective: Compare drug metabolism across different animal species and assess its relevance to human metabolism.

b. Methods: Conduct parallel studies in multiple species, analyzing similarities and differences in metabolite profiles.

c. Significance: Identify species-specific variations in metabolism, helping researchers interpret preclinical data and extrapolate to potential human outcomes.

4. Enzyme Involvement: Biotransformation studies investigate the specific enzymes involved in metabolizing the drug. Cytochrome P450 enzymes, for example, are often studied as they play a significant role in drug metabolism.

a. Objective: Investigate the specific enzymes responsible for drug metabolism.

b. Methods: Use enzyme inhibitors or inducers to selectively modulate the activity of specific enzymes, identifying their role in drug biotransformation.

c. Significance: Understand the contribution of different enzymes, especially cytochrome P450 enzymes, to predict potential drug interactions and optimize dosing regimens.

5. Drug-Drug Interactions: Understanding how a drug candidate interacts with other drugs during biotransformation is vital to anticipate potential drug-drug interactions in clinical settings.

a. Objective: Understand the proportion of the administered drug that reaches the systemic circulation in its unchanged form.

b. Methods: Evaluate factors such as absorption, distribution, metabolism, and excretion (ADME) to quantify bioavailability.

c. Significance: Determine the efficiency of drug absorption and potential limitations, guiding formulation adjustments for optimal bioavailability.

6. Bioavailability: Biotransformation studies contribute to understanding the bioavailability of the drug, which is the proportion of the administered drug that reaches the systemic circulation.
a. Objective: Understand the proportion of the administered drug that reaches the systemic circulation in its unchanged form.

b. Methods: Evaluate factors such as absorption, distribution, metabolism, and excretion (ADME) to quantify bioavailability.

c. Significance: Determine the efficiency of drug absorption and potential limitations, guiding formulation adjustments for optimal bioavailability.

7. Toxicology Considerations: Biotransformation studies also help in identifying potential toxic metabolites or pathways, contributing to the overall safety assessment of the drug candidate.

a. Objective: Identify and assess potential toxic metabolites or pathways.

b. Methods: Employ toxicological assays to evaluate the safety of metabolites and their impact on various organs and systems.

c. Significance: Contribute to safety assessments by identifying metabolites that may pose risks, allowing for refinement or discontinuation of drug candidates with unfavorable toxicity profiles.

Preclinical biotransformation studies involve the use of various equipment to analyze the metabolism of drug candidates in laboratory animals. The equipment used in these studies is designed to facilitate sample collection, analysis, and data interpretation.

Here are some types of equipment commonly used for preclinical biotransformation studies:

1. Animal Housing and Handling Equipment:
  • Animal Cages: Provide appropriate housing for laboratory animals.
  • Animal Identification Systems: Ensure proper identification of animals during the study.
  • Animal Handling Tools: Instruments for safely handling and restraining animals during dosing or sample collection.
2. Dosing Equipment:
  • Syringes and Needles: Administer precise doses of drug candidates to animals.
  • Oral Gavage Systems: Administer drugs via oral gavage for oral dosing studies.
  • Intravenous Catheters or Cannulas: Facilitate intravenous dosing for studies requiring direct administration into the bloodstream.
3. Sample Collection Equipment:
  • Blood Collection Tubes: Collect blood samples for subsequent analysis.
  • Centrifuges: Separate blood components, such as plasma or serum, from whole blood.
  • Tissue Collection Tools: Surgical instruments for harvesting tissues from animals post-drug administration.
4. Analytical Instruments:
  • Liquid Chromatography-Mass Spectrometry (LC-MS): Analyze samples for drug and metabolite concentrations with high sensitivity and specificity.
  • High-Performance Liquid Chromatography (HPLC): Separate and quantify compounds in biological samples.
  • Gas Chromatography-Mass Spectrometry (GC-MS): Analyze volatile compounds and metabolites. - Nuclear Magnetic Resonance (NMR): Provide structural information about drug molecules and metabolites.
5. Incubators and Shakers:
  • Drug-Metabolizing Enzyme Incubators: Maintain controlled conditions for in vitro studies simulating drug metabolism.
  • Shakers: Facilitate uniform mixing and incubation of samples during in vitro metabolism studies.
6. Safety Equipment:
  • Personal Protective Equipment (PPE): Ensure the safety of researchers handling potentially hazardous substances.
  • Fume Hoods: Provide ventilation for handling chemicals and protecting researchers from exposure.
7. Data Analysis and Documentation Tools:
  • Computers and Software: Analyze and interpret data obtained from analytical instruments.
  • Laboratory Information Management Systems (LIMS): Manage and document experimental data and sample information.
8. Quality Control Instruments:
  • Calibration Tools: Ensure the accuracy of analytical instruments.
  • Quality Control Standards: Monitor the performance of instruments and methods over time.
9. Storage Equipment:
  • Refrigerators and Freezers: Store biological samples at appropriate temperatures to maintain their integrity until analysis.
  • Sample Storage Systems: Archive samples for potential reanalysis or further investigations.

It’s important to note that the specific equipment used can vary depending on the nature of the preclinical biotransformation studies, the type of drug candidate, and the analytical methods employed. Additionally, adherence to safety regulations and ethical considerations is paramount in conducting these studies.

Overall, preclinical biotransformation studies provide valuable insights into the fate of a drug candidate within the body, aiding in the design of safe and effective drugs before progressing to human trials. These studies are typically conducted in accordance with regulatory guidelines to ensure the reliability of the data generated.

These aspects collectively contribute to a comprehensive understanding of the biotransformation of drug candidates, guiding decision-making in drug development and ensuring the safety and efficacy of drugs in subsequent clinical trials.

Links to Relevant Documents

Leadership

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Deborah J. Watson, Ph.D.

Group Leader – DMPK (In Vivo)
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Deborah J. Watson, Ph.D.

Group Leader – DMPK (In Vivo)
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Laura Gwinn

Research Associate I – DMPK (QWBA)
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Laura Gwinn

Research Associate I – DMPK (QWBA)

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