Effects of Resting Cryopreserved PBMCs on Viability, Subset Frequency, Chemokine Receptor Expression, and Cytokine Secretion
Monitoring cellular immune responses during drug development and clinical trials is critical for assessing the safety and efficacy of therapeutic interventions. Flow cytometry–based intracellular cytokine staining (ICS) and ELISpot assays are widely used for this purpose, each with distinct advantages and limitations.