Enhanced Metabolite Characterization of Therapeutic siRNAs Using Data Independent, Sequential Windowed Acquisition of all THeoretical (SWATH) Ions, LC-MS/HRMS Methods
Characterizing metabolites of drug-related small interfering RNAs (siRNAs) is essential for pharmacokinetic and safety assessment but remains challenging due to complex biotransformation pathways and the presence of isomeric species with multiple charge states.